Journal: World Journal of Gastroenterology
Article Title: Deciphering lactate metabolism in colorectal cancer: Prognostic modeling, immune infiltration, and gene mutation insights
doi: 10.3748/wjg.v31.i25.107478
Figure Lengend Snippet: Expression levels of biomarkers in colorectal cancer. A: Immunohistochemistry results demonstrating the expression level of histone H3 lysine 18 lactylation in colorectal cancer (CRC) tissues and paracancerous tissues; B: Immunofluorescence results of histone H3 lysine 18 lactylation levels in tumor tissues and paracancerous tissues; C: Western blot results demonstrating the protein expression levels of mitochondrial-dihydronicotinamide adenine dinucleotide dehydrogenase subunit 2 (MT-ND2), acyl-CoA dehydrogenase very-long-chain (ACADVL), solute carrier family 2 member 3 (SLC2A3), basic leucine zipper transcription factor ATF-like (BATF), RNA binding motif protein 3 (RBM3), inducible T cell costimulatory (ICOS), leptin receptor overlapping transcript-like 1 (LEPROTL1) in CRC tissues/paracancerous tissues, and normal colon cell/CRC cell lines (SW620, HCT116, WS480); D: The quantitative real-time fluorescence polymerase chain reaction results demonstrating the expression levels of MT-ND2, ACADVL, SLC2A3, BATF, RBM3, ICOS, and LEPROTL1 in CRC tissues and paracancerous tissues; E: The quantitative real-time fluorescence polymerase chain reaction results demonstrate the expression levels of MT-ND2, ACADVL, SLC2A3, BATF, RBM3, ICOS, and LEPROTL1 in normal colon cell and CRC cell lines (SW620, HCT116, WS480). c P < 0.001, compared with normal group. H3K18 La: Histone H3 lysine 18 lactylation; MT-ND2: Mitochondrial-dihydronicotinamide adenine dinucleotide dehydrogenase subunit 2; ACADVL: Acyl-CoA dehydrogenase very-long-chain; SLC2A3: Solute carrier family 2 member 3; BATF: Basic leucine zipper transcription factor ATF-like; RBM3: RNA binding motif protein 3; ICOS: Inducible T cell costimulatory; LEPROTL1: Leptin receptor overlapping transcript-like 1; FHC: Normal colon cells.
Article Snippet: Primary antibodies were: Anti-leptin receptor overlapping transcript-like 1 (anti-LEPROTL1) antibody (TA331958, Origene, MD, United States), anti-inducible T cell costimulator (anti-ICOS) antibody (AP13108PU-N, Origene, MD, United States), anti-RNA-binding protein 3 antibody (EPR6061, Abcam, United Kingdom), anti-basic leucine zipper transcription factor ATF-like (BATF) antibody (EPR21911-55, Abcam, United Kingdom), anti-solute carrier family 2 member 3 (anti-SLC2A3) antibody (LS-B7024-50, Lifespan Biosciences, WA, United States), anti-acyl-CoA dehydrogenase very-long-chain (anti-ACADVL) antibody (AP32801PU-N, Origene, MD, United States), anti-mitochondrial-dihydronicotinamide adenine dinucleotide dehydrogenase subunit 2 (anti-MT-ND2) antibody (LS-B15632-50, Lifespan Biosciences, WA, United States), anti-glyceraldehyde-3-phosphate dehydrogenase antibody (LS B4075, Lifespan Biosciences, WA, United States).
Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Western Blot, RNA Binding Assay, Fluorescence, Polymerase Chain Reaction